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Asian Journal of Plant Sciences
Year: 2004  |  Volume: 3  |  Issue: 2  |  Page No.: 196 - 199

In vitro Rooting and Ex vitro Plantlet Establishment of BARI Banana 1 (Musa sp.) as Influenced by Different Concentration of IBA (Indole 3-butyric Acid)

M.M.H. Molla, M. Dilafroza Khanam, M.M. Khatun, M. Al-Amin and M.A. Malek    

Abstract: Shoot tip culture of BARI banana 1 (Musa sp.) were cultured on MS medium supplemented with 5.0 mg L-1 BAP for shoot proliferation. Well-developed shoots were used for rooting. Among the six different concentrations of IBA (0.1, 0.2, 0.3, 0.4, 0.5, 0.6 mg L-1) with half strength MS medium, a good number of healthy roots were produced on half MS+0.5 mg L-1 IBA (7.86) followed by half MS+0.6 mg L-1 IBA (6.89) and half MS+0.4 mg L-1 IBA (6.31) with the weight of 0.85, 0.83 and 0.77 g plantlet-1, respectively. However, 95-100% plantlets were survived when they were transferred to small plastic pots after 15-20 days in vitro culture on half MS medium supplemented with 0.4–0.6 mg L-1 IBA and 7 days hardening at room temperature.

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