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Asian Journal of Plant Sciences
Year: 2003  |  Volume: 2  |  Issue: 12  |  Page No.: 913 - 915

Mineral Constituents of Culture Medium for Lipase Production by Rhizopus oligosporous Fermentation

Umar Farooq Awan, Kiran Shafiq, Shazia Mirza, Sikander Ali, Asad-ur- Rehman and Ikram-ul- Haq    

Abstract: Solid-state fermentation for lipase production was carried by Rhizopus oligosporous ISUUV-16. Almond meal was used as basal substrate with distilled water as moistening agent at substrate to diluent ratio of 1:0.7. Fermentation was carried out at 30oC and culture was incubated for 48 h. Maximum lipase activity under solid-state condition was obtained when Tween 80 at the level of 0.5 % was used as additional carbon source. Best organic nitrogen sources for optimal lipolytic activity was ammonium sulfate (4 %). This study would improve the further optimization steps on the bioprocess development track.

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