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Asian Journal of Plant Sciences
Year: 2003  |  Volume: 2  |  Issue: 5  |  Page No.: 425 - 430

A Phenylalanine Ammonia-lyase Gene from Asparagus: cDNA Cloning, Sequence and Expression in Response to Wounding

Pankaj Kumar Bhowmik, Toshiyuki Matsui, Haruo Suzuki and Yusuke Kosugi    

Abstract: An asparagus (Asparagus officinalis L. cv. Welcome) cDNA clone coding the enzyme phenylalanine ammonia-lyase (PAL) was isolated from a cDNA library prepared from asparagus spears using reverse transcription- polymerase chain reaction (RT-PCR). The partial cDNA clone encodes an mRNA of 527 bp and the derived amino acid sequence is highly homologous to PAL from rice, maize and barley. Northern blot analysis showed increase level of pAS-ACO mRNA until 24 hour at 200C, which coincided well with PAL activity and fiber development, suggesting that the increase might be a response to the wounding associated with harvest.

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