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Asian Journal of Plant Sciences
Year: 2003  |  Volume: 2  |  Issue: 1  |  Page No.: 102 - 107

Glutamine Synthetase in Harvested Asparagus Spears:Changes in Activity and Gene Expression During Storage

Pankaj Kumar Bhowmik, Toshiyuki Matsui, Haruo Suzuki and Yusuke Kosugi    

Abstract: Glutamine synthetase (GS; EC 6.3.1.2.) has a central role in plant nitrogen metabolism and is a key enzyme in the assimilation of ammonia. Asparagus (Asparagus officinalis L.) spear quality deteriorates rapidly after harvest and is associated with an increase in the ammonia content of the spear tip. In order to define the factors contributing to postharvest deterioration of asparagus spears, the changes in GS activity and gene expression in the top and bottom portions of asparagus spears were examined during storage. GS enzyme activity declined throughout the 96 h postharvest period in both top and bottom portion. Northern blot analysis showed that the level of transcript of GS decreased in top and bottom portion during storage. Expression of GS gene in top portion was observed more clearly but it was barely detectable in bottom portion that might be due to the lower activity of GS in bottom portion.

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