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American Journal of Plant Physiology

Year: 2014  |  Volume: 9  |  Issue: 1  |  Page No.: 16 - 23

Low Cost Material Enhanced the in vitro Regeneration and Micro Propagation of Medicinal Sand Dune Plant Species Ipomoea Pes-caprae (L.) R. Br.

P. Thirunavukkarasu, T. Ramanathan, G. Umamaheshwari, V. Manigandan and P. Dinesh

Abstract

Ipomoea pes-caprae is a sand dune plant commonly used as folklore medicine for fisherman communities based on the traditional knowledge. Little information is available on the feasibility of applying micropropagation techniques for production of dune and marsh species. The main aim of this study is to increase the callus induction and shoot generation of sand dune plant Ipomoea pes-caprae with easily available low cost natural materials. Coconut water is the rich source of carbohydrate and other nutrients which enhance callus and plant regeneration. In our present study, we tried different type of MS medium (Full and half strength) with coconut water at three different percentage (10, 15, 20%) and different concentration of plant growth regulators for callus induction and shoot regeneration. Well-developed callus inoculated in full and half strength MS medium with different concentration of CW and plant growth regulators. The best results were accomplished with half strength MS medium with 15% coconut water and with 2, 4-D and IAA 0.7 mg L-1 concentration which shows better callus induction and shoot regeneration. Young shoot and root developed plants transferred to green house and then followed to soil.

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