Phytochemical Investigation with Assessment of Cytotoxicity and Antibacterial Activities of the Ethanol Extract of Elaeocarpus serratus
The present study was designed to detect the presence of phytochemical compounds and to evaluate the cytotoxic profiles and antibacterial activities of the ethanol extract of the leaves of Elaeocarpus serratus. The phytochemical investigation was done according to the standard procedures. The phytochemical analysis of the extract showed the presence of alkaloid, glycoside, tannin, saponin, flavonoid and carbohydrate. The antibacterial tests of the plant extract and the standard drug, Levofloxacin were conducted against eight bacterial strains using agar disc diffusion technique. The extract at 500 μg disc-1 produced significant zone of inhibition against Bacillus subtilis (25.82±0.61mm), Bacillus megaterium (30.52±0.43 mm), Pseudomonas aeruginosa (20.35±0.51), Salmonella typhi (32.95±0.39 mm) and Vibrio cholerae (16.93±0.14) but no zone of inhibition was detected against Staphylococcus aureus, Escherichia coli and Shigella dysenteriae. The obtained results were also compared with the standard drug, Levofloxacin used at the concentration of 10 μg disc-1. Subsequently, the cytotoxicity test of the plant sample was performed using brine shrimp lethality bioassay which showed lethality against the brine shrimp nauplii with LC50 141.25 and LC90 870.96 μg mL-1. Finally, it was concluded that the ethanol extract of the leaves of Elaeocarpus serratus possessed significant antibacterial and cytotoxic properties.
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