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American Journal of Plant Physiology
Year: 2011  |  Volume: 6  |  Issue: 2  |  Page No.: 91 - 98

Study of Physiological Potentiality of Callus and Plant Regeneration through Somatic Embryogenesis in Rice Variety Swarna

Sananda Mondal, R.P. Singh, Chaitali Sen and Bandana Bose    

Abstract: The regeneration protocol was developed from dehusked and sterilized seeds of rice variety Swarna (MTU 7029) to study callus induction, callus growth rate and regeneration potentiality. Different ranges of 2,4-D (1-4 mg L-1) and combinations of 6-Benzyl Aminopurine (BAP) (2-4 mg L-1) and α-Naphthalene Acetic Acid (NAA) (1 mg L-1) were used for callus induction, somatic embryo formation and plantlets regeneration respectively. Ninty six percent callus induction efficacy was found with 4 mg L-1 2,4-D. Whereas the high frequency of plantlets regenerated from embryogenic calli were observed in the combination of 4 mg L-1 BAP and 1 mg L-1 NAA; the embryogenic calli obtained from 2 mg L-1 2,4-D concentration performed better in this media (4 mg L-1 BAP and 1 mg L-1 NAA) than the other three used concentrations of 2,4-D. Physiological potentiality of 45 days old calli in terms of fresh and dry weights and proline content were showed a positive correlation. However, 4 mg L-1 2,4-D in medium showed low regeneration capacity of calli.

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