Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
The American Journal of Physiology - Cell Physiology
Year: 2010  |  Volume: 298  |  Issue: 5  |  Page No.: 1253 - 1264

Odors activate dual pathways, a TRPC2 and a AA-dependent pathway, in mouse vomeronasal neurons

P Zhang, C Yang and R. J. Delay    


Located at the anterior portion of the nose, the paired vomeronasal organs (VNO) detect odors and pheromones. In vomeronasal sensory neurons (VSNs) odor responses are mainly mediated by phospholipase C (PLC), stimulation of which elevates diacylglycerol (DAG). DAG activates a transient receptor potential channel (TRPC2) leading to cell depolarization. In this study, we used a natural stimulus, urine, to elicit odor responses in VSNs and found urine responses persisted in TRPC2–/– mice, suggesting the existence of a TRPC2-independent signal transduction pathway. Using perforated patch-clamp recordings on isolated VSNs from wild-type (WT) and TRPC2–/– mice, we found a PLC inhibitor blocked urine responses from all VSNs. Furthermore, urine responses were reduced by blocking DAG lipase, an enzyme that produces arachidonic acid (AA), in WT mice and abolished in TRPC2–/– mice. Consistently, direct stimulation with AA activated an inward current that was independent of TRPC2 channels but required bath Ca2+ and was blocked by Cd2+. With the use of inside-out patches from TRPC2–/– VSNs, we show that AA activated a channel that also required Ca2+. Together, these data from WT and TRPC2–/– mice suggest that both DAG and its metabolite, AA, mediate excitatory odor responses in VSNs, by activating two types of channels, a TRPC2 and a separate Ca2+-permeable channel.

View Fulltext    |   Related Articles   |   Back
  Related Articles

No Article Found
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility