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American Journal of Immunology

Year: 2005  |  Volume: 1  |  Issue: 1  |  Page No.: 31 - 36

Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)

Lesley Maurice Bilung, Son Radu, Abdul Rani Bahaman, Raha Abdul Rahim, Suhaimi Napis, Cheah Yoke Kqueen, Chandrika Murugaiah, Yousr Abdul Hadi, Tunung Robin and Mitsuaki Nishibuchi

Abstract

Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains.

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