Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
American Journal of Food Technology
Year: 2007  |  Volume: 2  |  Issue: 1  |  Page No.: 12 - 20

Polyhydroxyalkanoates Production by Recombinant Escherichia coli Using Low Cost Substrate

Gustavo Graciano Fonseca and Regina Vasconcellos Antonio    

Abstract: Polyhydroxyalkanoates (PHAs) are thermoplastic, biodegradable polyesters, synthesized by some bacteria from renewable carbon sources. However, its application is limited by the high cost of production. To reduce these costs, recombinant strains that use diverse carbon sources have been developed. In this study, it was studied PHAs production by recombinant Escherichia coli (DH10B and JM10), harboring the structural genes of the polyhydroxyalkanoate synthases of Pseudomonas aeruginosa, using hydrolyzed corn starch and soybean oil as substrate, cheese whey as supplement and acrylic acid as fatty acids β-oxidation inhibitor. Their effect on the cell mass and the PHA content had been evaluated through an experimental design 24. The best results had been obtained with DH10B strain: Dry cell weight of 1.02 g L-1 and 23% of PHA (9 mol% 3-hydroxybutyrate, 4.5 mol% 3-hydroxyoctanoate, 30.7 mol% 3-hydroxydecanoate and 55.8 mol% 3-hydroxydodecanoate), in mineral media containing 5% of hydrolyzed corn starch, 5% de cheese whey and 5% of soybean oil, beyond 1 mM of acrylic acid.

Cited References   |    Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility