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American Journal of Drug Discovery and Development
Year: 2017  |  Volume: 7  |  Issue: 1  |  Page No.: 25 - 32

Antioxidant Activity of Polyphenolic Flavonoid of Stem of Nicotiana tabacum

Yash Sharma, Anshita Nagar, Nupur Sinha Srivastava and Kumud Bala    

Abstract: Background and Objective: Phytochemical constituents of herbal extracts has shown positive response of antioxidant activity. The purpose of the present study was to investigate the antioxidant activity of polyphenolic flavonoids in the ethanolic extract of stem of Nicotiana tabacum. Materials and Methods: Flavonoids contents were determined by both thin layer chromatography and aluminium chloride colorimetric method, where as phenolic content was determine by Folin-Ciocalteau method. Antioxidant activity was observed by determining the electron transfer (DPPH free radical scavenging), enzymatic biochemical assay (SOD and GST activity) and nonenzymatic biochemical assay (GSH content and lipid peroxidation). Results: Flavonoid and phenolic content were found to be present in the ethanolic extract i.e., 12.5±0.1322 mg QE g–1 of extract and 1133.25±0.02 mg QE g–1 of extract, respectively. It was found from the present study that 0.4 mg mL–1 of ethanolic extract has shown highest level of SOD, GST, GSH and MDA content. The HPLC analysis of ethanolic extract has also revealed the presence of flavonoids in them as compared to standard quercetin. Conclusion: This can be concluded from the present study that ethanolic extract of stem of Nicotiana tabacum has shown the presence of polphenolic flavonoids and antioxidant level can be utilized as traditional herbal remedy.

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