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Asian Journal of Biological Sciences
Year: 2018  |  Volume: 11  |  Issue: 4  |  Page No.: 197 - 202

Genetically Modified Organism Using ABA GA3 and IAA Hormone: Regulated Gene Expression

A.B.M. Sharif Hossain and Musamma Muddin    

Abstract: Background and Objectives: Genetically Modified (GM) technology retains an important role to produce GM organism (GMO) and GMO derived food. The study was carried out to investigate GM seedless pumpkin, seedless ladies finger and dwarfed peach plant employing different concentration of GA3 (100 and 150 ppm) and ABA (2000 ppm) hormones and its related gene expression. Materials and Methods: The treatments were used in expt. 1 as water control and abscisic acid (ABA) 2000 ppm applied to the partially peach barked tissue. Injection method was used in expt. 2 to make seedless pumpkin. Star fruit flower buds were dipped for 2 weeks with GA3 150 ppm and water control in expt. 3. The concentration of IAA 200 ppm were applied to the ladies finger's flower by injecting in Expt. 4. Results: From the results it was observed that bark tissue, shoot and root were inhibited by ABA hormone. Whereas in control plant, there was no inhibition occurred. In expt. 2, it had been shown that fruit weight was higher in the GA3 treated fruit than in control. However, seed number and per seed weight were higher in the control than the GA3 treated pumpkin. Glucose, fructose potassium and calcium content were found higher in the GA3 treated pumpkin than the control. In expt. 3, fruit weight was higher in the GA3 treated fruit than in control. However, seed number was higher in the control than the GA3 treated fruit. In expt. 4, the maximum pod length, TSS and vitamin C were obtained in IAA 200 mg L–1 compared to the control. The highest seedless pod was found in IAA 200 mg L–1. Conclusion: From the results it has been seen that maximum seedless pod was 100%. Positively gene was expressed by ABA 2000 ppm and GA3 150 ppm.

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