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Asian Journal of Biological Sciences
Year: 2013  |  Volume: 6  |  Issue: 6  |  Page No.: 306 - 311

Serodiagnosis of Immune Response Exhibited by 24,000 g Fraction in Rodent Malaria

Nisha Devi and H.S. Banyal    

Abstract: Immunity to malaria can be induced by experimental infection in rodents. A large number of malaria antigens have been identified having their role in protective immune responses to malaria. In this study, mice immunized with soluble antigens of 24,000 g sedimented fraction exhibited strong humoral immune response and this was evaluated by serodiagnostic assays. When 24,000 g fraction were subjected to SDS-PAGE, showed protein bands with molecular weights ranging from 24-80 kDa. It appears that some of these proteins act as target antigens. The immune sera obtained from immunized mice were subjected to ELISA, IFA and in vitro invasion inhibition assay. The antibody titres ranged from 1:2048-1:16384 in immune sera.

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