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Asian Journal of Biological Sciences
Year: 2013  |  Volume: 6  |  Issue: 4  |  Page No.: 214 - 220

An Improved Micropropagation Protocol for Plumbago zeylanica L. An Important Medicinal Plant

S. Antony Ceasar, M. Ayyanar and S. Ignacimuthu    

Abstract: An improved protocol was developed for the micropropagation of Plumbago zeylanica L. from nodal explants. The best response of shoot induction was observed on Murashige and Skoog (MS) basal medium supplemented with 1.0 mg L-1 thidiazuron (TDZ) and 1.0 mg L-1 Kinetin (KN). The regenerated shoots rooted best on half strength Murashige and Skoog basal medium containing 1.0 mg L-1 indole-3-butyricacid (IBA). In vitro propagated plants were transferred to soil with 90% survival rate. The in vitro obtained plants did not show any detectable morphological variation compared to the normal plants. This system will help in the mass production of Plumbago zeylanica and other medicinally important plants.

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