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Asian Journal of Biological Sciences
Year: 2011  |  Volume: 4  |  Issue: 6  |  Page No.: 514 - 519

Presence of an Intron Indicated in a Candida krusei Tec1 Gene: A Preliminary Report

Okungbowa Francisca I., Pal Kalidas, Chowdhury Rukhsana and Okungbowa Michael O.A.    

Abstract: A Candida krusei clinical isolate was investigated for the presence of Tec1 (a C. albicans virulence gene) using nucleotide amplification-based techniques. Amplification of genomic DNA did not show the presence of Tec1 gene but the gene was expressed when Reverse-Transcriptase-Polymerase Chain Reaction (RT-PCR) was used. This is indicative of the presence of one or more introns in Tec1 gene of the C. krusei which could play a role in chemotherapy. This is a preliminary report that requires further investigation.

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