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Asian Journal of Biological Sciences
Year: 2010  |  Volume: 3  |  Issue: 2  |  Page No.: 85 - 91

Use of Open Pulled Straw as a Carrier in Vitrification of Metaphase II Oocyte in Mice

F. Rahim and G. Saki    

Abstract: This study taking to account the limitations of studies in this area, aimed to compare the survival rate, fertilization capacity and developmental capacity of oocyte that cryo-preserved in open pulled and conventional straw. Total 90 females NMRI mice were used in this research. First the male mice were scarified then the caudal part of epididymis was isolated. By creating cuts in caudal part of epididymis, sperms were isolated and put in T6 medium + 5 mg mL-1 BSA. Later on the sperms were incubated under 37°C and 5% CO2 for 2 h. Then sperm were added to living control, cryo-preserved in conventional and open pulled straws oocytes. After 4-6 h changing medium, two nuclear and two-cell embryos were evaluated 8 and 24 h after addition of the sperms, respectively. Comparing the two groups shows that the vitrified oocyte of open pulled straw method had better and significant different survival rate than conventional method (p = 0.001). Furthermore, between open pulled group and conventional group also there was significant different in term of fertilization rate (p = 0.001). The developmental capacity of open pulled straw group was significantly higher that conventional straw group (p = 0.002). Oocyte cryo-preservation, if consistent and successful, offers a way to avoid the complications of routine IVF therapy. Oocytes may need to be cryo-stored in the event of unforeseen non-production of sperm during IVF therapy, allowing a more measured consideration of donor sperm use or other means of sperm retrieval.

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