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Asian Journal of Biological Sciences
Year: 2008  |  Volume: 1  |  Issue: 1  |  Page No.: 19 - 25

Variation of High-Molecular-Weight Glutenin Subunits and Gliadin in T.aestivum ssp. macha

Li-Juan Xiong, Wei Li, Yu-Ming Wei and You-Liang Zheng    

Abstract: In order to exploit new genetic resources and provide fundamental materials for the breeding improvement of bread wheat quality, genetic variation of high-molecular-weight glutenin subunits and gliadin in 29 macha wheat accessions were observed by acidic polyacrylamide-gel electrophoresis and sodium dodecyl sulphate polyacrylamide-gel electrophoresis. Nine HMW-glutenin subunits alleles and 9 combinations were identified. Subunit null (82.8%), 7+8 (53.3%) and 2+12 (82.8%) scored the highest frequency at Glu-A1, Glu-B1 and Glu-D1 loci, respectively. In addition, subunits 7+9 (23.3%) was also found at higher frequency. A total of 49 gliadin bands and 28 patterns were detected and the polymorph among all accessions was identified in most of bands (97.96%). Furthermore, all materials could be clustered into three major groups based on genetic similarity coefficient. The results indicated that the variations of gliadin among macha accessions were not associated with their geographic origins.

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