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American Journal of Biochemistry and Molecular Biology
Year: 2018  |  Volume: 8  |  Issue: 1  |  Page No.: 1 - 9

Assessment of Biochemical Parameters in Heavy Metal-stressed Crop of Mung Bean [Vigna radiata (L.) R. Wilczek]

Nidhi Saini and Sarika Gupta    

Abstract: Background and Objective: Every year, a significant portion of agricultural yield is hampered due to increasing level of heavy metals and other chemical in the soil leads in low productivity and sometimes no growth at all. This chemical seriously impairs the growth of the plants and affects the overall productivity. The remedy for this problem lies towards microbial degradation of these compounds. The study aimed to elucidate the alterations in the physiological parameters, biochemical parameter and protein profile in stressed seedlings with respect to control. Materials and Methods: The present study involved the impact of heavy metals stress (zinc and cadmium) on mung seedlings under in vitro conditions. The seeds were of research grade and the same size. The entire study was conducted in sets of three (triplicates) and the data were statistically analyzed, represented the mean of the dataset. Results: Data revealed that the water agar seedling symptom test the severity of the effect on the seedlings was in the order as cadmium followed by zinc. During metabolic analysis, initially at low concentration there was an increase and a sharp decrease at higher concentration in the photochemical contents. Protein profiling exhibited a band of 97.4 KDa protein in all the stress induced plant with respect to the control. So this might be the protein that is most probably a part of the stress machinery in the plant. Conclusion: As it is rapidly progressing towards the development, the problems associated with it will become increasingly complex in near future. Hence, it is the time to take a precautionary step ahead so that the situation can be handled in future and find a solution for it. This study is initiative to deal with the present problems of the society to have a better and healthy future.

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