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American Journal of Biochemistry and Molecular Biology
Year: 2017  |  Volume: 7  |  Issue: 2  |  Page No.: 65 - 78

Phenotypic and Molecular Characterization of some Virulence Factors in Multidrug Resistance Escherichia coli Isolated from Different Clinical Infections in Iraq

Ahmed Abduljabbar Jaloob Aljanaby and Qassim Mmuhsin Hashim Alfaham    

Abstract: Objective: The aim of this study was to detect 10 virulence genes in 50 Escherichia coli strains isolated from patients with different infections. Methodology: Different phenotypic assays and multiplex PCR were used to detect virulence genes. Antimicrobial susceptibility testing was performed to 14 antimicrobials types by disk diffusion method. Results: The most common virulence gene was feoB (98%) and at lower prevalence were genes eaeA and stx1 (4%). Escherichia coli strains were highly resistant to most antimicrobials. Conclusion: Escherichia coli strains isolated from patients with bacteremia were highly virulence and more resistance to antimicrobials than those isolated from other sites.

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