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American Journal of Biochemistry and Molecular Biology
Year: 2013  |  Volume: 3  |  Issue: 3  |  Page No.: 293 - 303

Biopolymer Production Using Pseudomonas Sp. (MTCC) and its Application in Agriculture

D. Latha, Sandya, Shahina and Rekha    

Abstract: Biodegradable polymers derived from biomass have recently obtained much attention from the public because they are synthesized from renewable raw materials. Polyhydroxybutyrate (PHB) is one such biopolymer. The objective of study is to identify PHB accumulating Pseudomonas sp. which is obtained from Microbial Type Culture Collection, Chandigarh. All the 5 species showed positive result for PHB accumulation by rapid screening method using Sudan black B stain and Microscopy. Optimization of time as carried out and it showed that 48 h of incubation found to be optimum for maximum accumulation in Pseudomonas aeruginosa. Biochemical analysis of various cheap substrates such as agricultural residues (rice bran and wheat bran), forestry residues (saw dust) and industrial residues (activated sludge) which can be used as a carbon source showed that activated sludge has less nitrogen, lignin and phenolic content which enhanced maximum accumulation than the other residues in P. aeruginosa. Biochemical analysis of PHB by Nuclear Magnetic Resonance (NMR) spectrophotometer was carried out and the peaks correspond to the chemical group of PHB. Agricultural application showed that PHB enhanced germination and overall plant growth in leguminous plants.

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