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American Journal of Biochemistry and Molecular Biology
Year: 2011  |  Volume: 1  |  Issue: 4  |  Page No.: 368 - 374

Conformational Analysis of Synthesized Ovine Kisspeptin 13 using Circular Dichroism Spectroscopy

Vijay Kumar Saxena, Sameer Srivastava, Satish Kumar and S.M.K. Naqvi    

Abstract: Kisspeptin, also called as metastin, is the cognate ligand GPR-54 (G-coupled receptor) which was previously an orphan receptor. Kisspeptin consists of 145 amino acids and its biological activity can be localized to the C-terminal segment which is cleaved into C-10, C-13 and C-14 segment. Kisspeptin-GPR-54 interaction stimulates GnRH secretion and has been shown to be essential for the initiation of the pubertal LH surge. A 13 amino acid derivative of kisspeptin was synthesized by solid phase peptide synthesis using F-moc strategy and it was purified using RP-HPLC. The CD spectra of the peptide (1 mg mL-1) was evaluated in water, 50% and 75% TFE. The CD spectra of kisspeptin 13 in the water mainly exhibited random coil structure with no significant alpha helical or beta sheet content. Increasing concentration of TFE could cause slight induction of both beta sheet and alpha helix structures but still disordered random coil being the major contributor to the conformation. Predominantly disordered structure of kisspeptin 13 as elucidated by the experiment may explain its multifunctional role and non putative receptor binding and may help to develop a few agonists for mimicking the therapeutic action for future drug use.

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