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Asian Journal of Biotechnology
Year: 2020  |  Volume: 12  |  Issue: 2  |  Page No.: 65 - 74

Infusion Extraction of Toxin from Chili Pepper (Capsicum baccatum) for Bedbug Protection

Abenezer Wendimu and Wondimagegnehu Tekalign    

Abstract: Background and Objective: The developing trend of resistance in bed bugs toward synthetic insecticidal agents makes their management extremely difficult. So, there is an urgent need to develop new approach in the protection of bedbugs consist of using native plants with insecticidal value. The present experiment was conducted to protect bed bugs through the use of Capsicum baccatum (chilli pepper) extract. Materials and Methods: The experiment was conducted to extract toxin from Capsicum baccatum for bedbug protection. The extract was prepared in 3 different concentrations in liquid form viz. 14.2, 16 and 18.8% as a direct admixture of salt. Adult bedbugs were collected from student’s dormitory The collected insects were allowed to mate for 3 days and eggs from ovipositor site were collected. The extract was sprayed on adult bedbugs designed in experimental block with 3 treatment groups. The efficiency of acute toxicity of the extract was checked on the percentage of adults mortality in control with percentage control and inhibition of adult emergence was checked by percentage of adult emergence inhibition in control [IE %]. Bonferroni correction test was used to adjust for multiple comparisons of the blocks at 95% confidence interval [CI]. The experimental design for mortality and emergence test was randomized block design. Results: The result revealed that the extract was 100% effective at killing bedbugs and inhibiting adult emergence in all levels of concentration [CL] compared to treatments under control with significant mean difference (p<0.05). Conclusion: Generally, the study confirmed that the extract pose talented potential in protecting bedbugs and adult emergence inhibition only in small scale applications based on the demonstrated efficacy and reduced risk potential on non-target organisms and ecology.

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