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Asian Journal of Biotechnology
Year: 2020  |  Volume: 12  |  Issue: 2  |  Page No.: 127 - 135

Analysis of Yardlong Bean (Vigna unguiculata Subsp. sesquipedalis) Genetic Diversity using RAPD Markers

Muhammad Shahidul Haque, Afifa Azad, Nihar Ranjan Saha and Muhammad Monirul Islam    

Abstract: Background and Objective: Yardlong bean is a well-known vegetable because of its long, slender, succulent pods and its protein content. In the present study genetic variation and relationships among yardlong bean genotypes were investigated thorough RAPD marker technique. Materials and Methods: Thirty six genotypes of yardlong bean were planted in the field to characterize their genetic relatedness and identify variation at molecular level. Sixteen decamer RAPD oligonucleotide primers were screened and out of these, 10 primers were chosen for further evaluation. Results: Altogether 94 RAPD bands were scored of which 82 were polymorphic. The amplified product size ranged from 150-900 bp. The primer OPB-08 amplified maximum number of polymorphic bands. The average number of polymorphic bands/primer was 8.2. The frequency of polymorphism ranged from 0.0278-0.9722. Nei’s pair-wise genetic distance ranged from 0.055-0.83. A UPGMA dendrogram of genetic similarity was constructed based on 82 polymorphic bands obtained from 10 primers. The UPGMA dendrogram divided the studied individuals into four main clusters. BD-1528 and BD-1524 accession formed distinct cluster (I and II, respectively). Most of the genotypes were observed in cluster IV (29 genotypes) which was further sub-divided into two sub-clusters which were subdivided into sub-sub-clusters making the genotypes diverse. Accessions collected from Bangladesh Agricultural Research Institute, showed significant diversification as they were widely distributed in 4 clusters. Conclusion: The variability among the genotypes has the potential to be used in future plant breeding program as well as for commercial production.

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