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Asian Journal of Biotechnology
Year: 2012  |  Volume: 4  |  Issue: 1  |  Page No.: 30 - 37

Assessment of Genetic Relationship among 15 Citrus Fruits Using RAPD

M.A. Maya, M.G. Rabbani, M.G. Mahboob and Y. Matsubara    

Abstract: Genetic relationship among 15 citrus fruits (one from the genus Poncirus and the rest from the genus Citrus) was assessed using Randomly Amplified Polymorphic DNA (RAPD) markers. Seven primers were selected after screening of 39 decamer primers which generated 91 clear and bright polymorphic bands. The Unweighted Pair-group Method using Arithmetic averages (UPGMA) dendrogram based on Nei’s genetic distance segregated 15 citrus fruits into 2 main clusters where Poncirus trifoliata alone formed one cluster and the rest 14 species grouped together into another cluster. Inter-species similarity index and the UPGMA dendrogram based on Nei’s genetic distance revealed that Citrus megaloxycarpa and C. limonia as the most closely related species among the 15 citrus fruits under study.

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