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Asian Journal of Biotechnology
Year: 2011  |  Volume: 3  |  Issue: 6  |  Page No.: 564 - 572

In-vitro Callus Development of Different Explants used for Different Medium of Terminalia arjuna

A. Arumugam and K. Gopinath    

Abstract: An efficient protocol for in vitro propagation of Terminalia arjuna is described by callus regeneration. Four different explants were used to establish callus to develop shoot and root regeneration method. MS (Murashiege and Skoog), LS (Linsmaier and Skoog) and B5 (Gamborg) basal medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D, 0.1-20.0 mg L-1), with combination of Naphthalene acetic acid (NAA 0.1-20 mg L-1), Indole-3-acetic acid (IAA 0.1-20 mg L-1) and Benzylaminopurin (0.1-20 mg L-1). Ms medium was found to be the most favorable for callus induction compare with LS and B5 media. Maximum number of callus regeneration was obtained on MS medium containing 2,4-D 3.0 mg L-1. The callus culture to develop the shoot and root initiation in MS basal medium substituted with 5 mg L-1 2, 4-D+0.01 mg L-1 Kinetin and 1.0 mg L-1 Gibberellic acid (GA3). The rooted shoot plantlet was transferred in to small plastic cups containing sterile vermiculate, sand and red soil in the ratio of 1:2:2 and were kept in a mist house. The regenerated plantlets were hardened in the greenhouse and successfully transferred in soil with 87% survival rate. This in vitro micro-propagation method with possibility of developing a new protocol was standardized the T. arjuna plant.

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