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Asian Journal of Biotechnology
Year: 2011  |  Volume: 3  |  Issue: 4  |  Page No.: 368 - 377

Genetic Diversity in Hexaploid Wheat Genotypes using Microsatellite Markers

A.H. Gorji and M. Zolnoori    

Abstract: The aim of this study was to molecularly characterize the thirty-eight wheat cultivars recommended for various regions of Iran and to assess mutual genetic distances, through the use of microsatellite markers. Polymorphic information content (PIC value) calculated to assess the informativeness of each marker ranged from 0.19 to 0.78 with a mean of 0.49.Twenty three Polymorphic Microsatellite Markers (PMM) delineated all 34 of the samples, revealing a total of 73 Simple Sequence Repeat (SSR) alleles, i.e., an average of 3.2 alleles per locus. Genetic distances among the 38 genotypes ranged from 0.11 (between genotypes S-83-20 and N-83-3 ) to 0.88 (between genotypes D-83-2 and M-83-5) the mean distance being 0.47. Thirteen groups were obtained by using the unweighted pair-group method with arithmetic means analysis (UPGMA) and fourteen through the Tocher method. Both methods produced similar clusters with one to thirteen genotypes per group. Twenty three polymorphic microsatellite markers delineated all 38 of the samples, revealing a total of 37 simple sequence reapt (ssr) alleles, i.e., an average of 3.1 alleles per locus. The results indicate that these tools may be used to protect intellectual property and for breeding and selection programs.

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