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Asian Journal of Biotechnology
Year: 2011  |  Volume: 3  |  Issue: 3  |  Page No.: 269 - 274

RAPD Fingerprinting and Demonstration of Genetic Variation in Three Pathogens Isolated from Mangrove Environment

M. Thangaraj, Vishruth Prem, T. Ramesh and A.P. Lipton    

Abstract: The aim of the study was to find the RAPD finger printing of Pseudomonas aeruginosa, Bacillus circulans and Serratia marcescens, using ten random primers. The RAPD-PCR produced reproducible electrophoretic band patterns only in seven primers. There were 67 species specific bands observed, among these, 25 bands in P. aeruginosa, 19 in B. circulans and 23 in S. marcescens. This study suggests that RAPD typing could be an additional rapid typing method for studying the epidemiology of these pathogens. Based on the RAPD data the genetic identity between S. marcescens and B. circulans was more (0.3803) than the other.

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