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Asian Journal of Biotechnology
Year: 2011  |  Volume: 3  |  Issue: 2  |  Page No.: 165 - 176

Profuse Microtillering of Androgenic Plantlets of Elite Indica Rice Variety IR 72

Bidhan Roy and Asit B. Mandal    

Abstract: The aim of this experiment was to induce profused microtillering of androgenic plantlets of elite indica rice (Oryza sativa L.) variety IR 72. Two microtillers were inoculated in each culture tube containing different medium and growth regulators. Various combinations of growth regulators were also used with each culture medium. Microtillering ability were noted on 45th day after inoculation of microtillers. MS medium with 4-8 mg L-1 of kinetin showed very fast microshooting with maximum of 94.7 shootlets per cultured shoot within 45 days of inoculation. Conversely, BAP at high dose (4-8 mg L-1) was found to exert inhibitory effects on shoot development, but induced large number of dormant embryos and pro-embryos. The caulogenic shoots that emerged from the base of the explants were successfully induced root on MS supplemented with 1 mg L-1 IAA. Evaluation of clonal fidelity of the microshoots through profiling of isozyme in respect of peroxidase and malate dehydrogenase and RAPD analysis was done to confirm true-to-type nature of the micropropagated plants. Microtillering and subsequent whole plant development may offer ample scope to develop large number of clones from a stock of choice for in vitro conservation, characterization and screening for diverse characters of agronomic importance. This approach may have far reaching consequences in transgenic development especially through direct bombardment of miniature shootlets en route biolistic and their subsequent multiplication en masse.

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