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Asian Journal of Biotechnology
Year: 2010  |  Volume: 2  |  Issue: 2  |  Page No.: 127 - 132

Microencapsulation of Saccharomyces cerevisiae using a Novel Sol-Gel Method and Investigate on its Bioactivity

A. Mellati, H. Attar and M.F. Farahani    

Abstract: Living Saccharomyces cerevisiae (S.c.) was microencapsulated with a Sol-Gel method using Tetra Methyl Ortho Silicate (TMOS) as a precursor so that it saved its bioactivity. A W/O emulsification technique was used. A vegetable oil was the organic phase for microencapsulation. Bioactivity of immobilized yeast in microcapsules was investigated, measuring the amount of CO2 released from culture as a result of fermentation. Production of CO2 by encapsulated yeast increased about 150% more than free yeast. Repetition of the tests has proved that microcapsules were saved their bioactivity during a month. On the other hand, particle size was decreased from 175 to 110 μm by increasing the mixer rate from 600 to 1200 rpm during gelation and smaller particles showed more bioactivity up to 30%.

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