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Asian Journal of Biotechnology
Year: 2009  |  Volume: 1  |  Issue: 1  |  Page No.: 29 - 36

Induction of Thermotolerance in Saccharomyces cerevisiae Strain(s) using Different Mutation Methods

S. Tork, W.K. Hegazy, T.M.A. El-kawokgy and O.G.A. El-Gebaly    

Abstract: The present study aimed to induce heat tolerant mutants in haploid Saccharomyces cerevisiae 5a FAIII strain by using different mutation methods. Three different mutation induction experiments were carried out to produce genetically stable heat tolerant Saccharomyces cerevisiae strains. The haploid strain 5a of the S. cerevisiae FAIII that has the highest growth rate was used throughout these experiments. Three thermo-tolerant mutants named as 5a1, 5a2 and 5a3 were obtained using N-methyl N-nitro N-nitrosoguanidine (MNNG), combination of ultra-violet radiation and hydroxylamine (UV+HA) treatments and spontaneous mutation, respectively. The mutants could tolerate 42 and 42.5°C but they couldn’t survive extended period of time at 43°C. The growth rate of the mutants indicated that they exhibited well growth at 42°C, while they exhibited slow growth rate at 42.5°C. Although (MNNG) induced thermotolerance mutation at high frequency, it was found that mutants induced following synergistic effect of (UV and HA) exhibited more stability. On the other hand, the spontaneous mutant exhibited the best growth rate at high temperature.

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