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Asian Journal of Biotechnology
Year: 2009  |  Volume: 1  |  Issue: 4  |  Page No.: 163 - 170

Isolation of Host-Specific Bacteriophages from Sewage Against Human Pathogens

Mahadevan M. Sundar, Nagananda G.S., Arijit Das, Sourav Bhattacharya and Sandeep Suryan    

Abstract: Bacteriophages have been found to be effective against a wide variety of pathogenic bacteria as they are highly host specific. The present study describes the isolation of bacteriophages effective against few human pathogens such as Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa. A total of five isolates of bacteria were obtained from the sewage water, sampled from the sewage treatment plant located at Jinke Park, Bangalore, India. Based upon the colony morphology, biochemical characterization and growth on selective media, the isolates were identified as Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella species and Shigella species. Out of the five different isolates three were sensitive to bacteriophages. The sensitive cultures belonged to the genera Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa. The phage filtrates, when spotted onto the lawn cultures of the respective host bacterium, resulted in the development of clear zones indicating the presence of lytic bacteriophages against the host bacteria. It was also found that each of the phages for E. coli and Salmonella typhi was only able to infect its original host bacterium, whereas, the phage for Pseudomonas aeruginosa was able to infect both Pseudomonas and E. coli. Studies of the morphology and characterization of these phages are currently being conducted. These isolated phages may hold a lot of promise as the first choice of prophylaxis (Phage Therapy) against nosocomial and secondary infections by deadly multi-drug resistant bacteria in the near future.

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