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Asian Journal of Biotechnology
Year: 2009  |  Volume: 1  |  Issue: 4  |  Page No.: 154 - 162

HPTLC and HPLC Analysis of Bioactive Phyllanthin from Different Organs of Phyllanthus amarus

A. Annamalai and P.T.V. Lakshmi    

Abstract: One of the most widely used herbs in Ayurvedic medicine is Phyllanthus amarus, which is predominantly grown in Indian subcontinent. Since, the market surveillance of herbal drugs is reported to contain minimum 0.5% of phyllanthin, demands increased production of this herb, which could be achieved by standard agrotechniques. Moreover, since, it is also desirable to check for the effect of these improved measures on the bioactive compound-phyllanthin, an effort was aimed at investigating a standard strategy to quantify the principle compound. Hence, a detailed investigation was adopted to provide a comparative quantification of phyllanthin through High Performance Thin Layer Chromatography (HPTLC) and High Performance Liquid Chromatography (HPLC) from different organs of P. amarus grown with improved agrotechniques. However, it revealed to be more effective through HPLC especially higher in the leaves as against the analysis through HPTLC. 

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