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Asian Journal of Biotechnology
Year: 2009  |  Volume: 1  |  Issue: 3  |  Page No.: 118 - 123

Influence of Cultural Conditions on Lipase Production in Candida albicans

Binn Jatta, M. Gunasekaran and N. Mohan    

Abstract: We studied the Lipase (LP) an enzyme which hydrolyses the ester bonds of triacylglycerols to yield glycerol and fatty acids in an opportunistic yeast human pathogen, Candida albicans (CA). The yeast was grown in Sabouraud dextrose broth and Lee synthetic medium at 25°C on a rotary shaker (100 rpm). At 24, 48 and 72 h of inoculation, cells were separated from the media and the intracellular and extracellular LP were measured from cell free homogenate and culture media, respectively. Lipase activity was determined by the rate of hydrolysis of olive oil emulsion by potentiometric titration. The influence of various factors such as growth, pH, temperature and media on the production of extra and intracellular Lipases (LP) has been studied. All the experiments were conducted at least twice and the analyses were carried out in triplicates. Candida albicans produced both extra and intracellular lipases in both the tested media. Although, LP was produced throughout the growth phase, maximum enzyme activity was detected at 24 h of growth. Optimum pH and temperature for the LP activity were 7 and 37°C, respectively.

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