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American Journal of Biochemistry and Biotechnology
Year: 2007  |  Volume: 3  |  Issue: 3  |  Page No.: 167 - 170

Platelet Derived Growth Factor-A mRNA Levels in Diabetic and Nondiabetic Subjects at Risk of Coronary Heart Disease

Salman A. H. Alrokayan    

Abstract: Coronary heart disease (CHD) is the number one cause of death in developed countries. We investigated the quantity of platelet derived growth factor-A mRNA (PDGF-A mRNA) among four different groups of diabetic and nondiabetic Saudi subjects (n = 40) at risk of CHD. Total RNA was derived from peripheral blood mononuclear cells (PBMN) and PDGF-A mRNA reverse transcribed into cDNA in the presence of internal standard (cRNA) and amplified by competitive reverse transcriptase polymerase chain reaction (cRT-PCR). The gel was silver stained after polyacrylamide gel electrophoresis (PAGE) and PDGF-A mRNA quantitated by scanning densitometry. We demonstrate a significantly high expression of PDGF-A mRNA in hyperlipidaemic and diabetic subjects (41 ± 4 x 104 copies/μg of RNA) compared to normal healthy individuals (29.3 ± 5 x 104 copies/μg of RNA). These findings suggest an important role of PDGF-A mRNA in progression of CHD among diabetics.

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