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American Journal of Biochemistry and Biotechnology
Year: 2005  |  Volume: 1  |  Issue: 2  |  Page No.: 107 - 110

Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) Callus

Ali Akbar Ehsanpour and Roya Razavizadeh    

Abstract: Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area; consequently, the study of plant responses to water stress is important. In this research UV-C radiation was used to induce physiological and genetic changes in alfalfa callus. In vitro grown calluses of M. sativa were exposed to UV-C for 0, 15, 30 and 60 min and then, were transferred to MS medium containing 0, 2, 5, 10, 20, 30% PEG. All explants were kept in the dark in the culture room. Calluses tolerate to osmotic stress were selected according to fresh weight. Results showed that radiation of UV-C for 60 min increased the osmotic tolerance and decreased the effect of drought stress.

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