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Asian Journal of Biochemistry

Year: 2020  |  Volume: 15  |  Issue: 2  |  Page No.: 42 - 49

Artemether-lumefantrine Improved Superoxide Dismutase Co-regulation Related Antioxidant Defense in Monosodium Glutamate-induced Oxidative Stress in Rats

Anthony Cemaluk C. Egbuonu, Ebere Mercy Chukwu, Wisdom Chibuzor, Emmanuel Obi, Nnaemeka Raymond Ezenwafor, Cynthia Uchechi Simon and Justina Utodinachi Oleghibe

Abstract

Background and Objective: Oxidative stress, co-regulated by Superoxide Dismutase (SOD) with its cofactors (Zn and Mg), could be synergistic when its respective Mediators Monosodium Glutamate (MSG) and Artemether-Lumefantrine (AL) are co-taken. Thus, the study estimated SOD activity, zinc and magnesium concentration variations in artemether-lumefantrine co-treated monosodium glutamate-assaulted rats’ serum and liver homogenate. Materials and Methods: Thirty rats acclimatized for two weeks were randomly grouped into six (n = 5) and for seven days exposed to feed and water (Group A, control). In addition, rats in Groups B, C, D, E and F, respectively received AL therapeutic dose, AL overdose (therapeutic dose ×5), MSG, 8000 mg kg1 b.wt., AL therapeutic dose plus MSG and AL overdose plus MSG. Data obtained from spectrophotometric the estimation of the studied parameters were subjected to analysis of variance (ANOVA) set at 95% confidence level. Results: The higher serum and liver homogenate zinc concentration (μg dL1) in all groups relative to MSG was particularly marked (264.91±1.89, 174.04±1.33; p<0.05) in AL overdose plus MSG-intoxicated rats. Similarly, AL overdose plus MSG-assaulted rats had a markedly higher (p<0.05) serum (6.88±0.10) Mg concentration (Mg dL1) compared to others, including control and MSG-intoxicated rats. Amid an unclear-cut change, SOD activity (IU L1) in the rats’ liver homogenate was highest (1.14±0.00, p<0.05) in MSG-treated rats relative to control. Conclusion: The study confirmed oxidative stress that overwhelmed the SOD-related antioxidant defense in MSG-assaulted rats unlike in rats exposed to AL irrespective of dose or co-MSG-intoxication. This perhaps implicated SOD as reserved antioxidant defense arsenal in possible AL or AL with co-MSG-intoxication adversity in rats’ serum and liver, provoking further studies.

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