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Asian Journal of Biochemistry
Year: 2014  |  Volume: 9  |  Issue: 2  |  Page No.: 114 - 118

Comparative Study in Relation to Protein and Protein Profiling of Rhizobium Inoculated Desi and Kabuli Chickpea (Cicer arietinum L.) Genotypes

Harendra Singh, Pratibha Singh, R.P. Singh and Mritunjay Tripathi    

Abstract: Fifteen genotypes of chickpea control and Rhizobium inoculated were investigated for protein content and genetic divergence based on seed protein profile using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Protein content of desi type was relatively higher than kabuli type. Protein content fifteen genotypes ranged from 18.76-23.58% in control, Rhizobium inoculated 19.83-24.54%. Desi and Kabuli genotype of chickpea Rhizobium inoculated gave some unique band in comparison to control genotype of chickpea. Desi genotypes gave some unique band in comparison to Kabuli genotypes of chickpea. Highest number of protein band found in PUSA 362, KWR 108 and followed by H82-2 and Phule G 5 whereas minimum number of protein bands were found in L550. It is clear from the result obtained that the chickpea genotype having highest number of protein band having highest quantity of protein. Maximum bands are of medium molecular weight followed by highest molecular weight and low molecular weight. Electrophoresis (SDS-PAGE) of seed storage protein can economically be used to assess, genetic variation and relation in genotypes. Thus, specific bands of seed storage protein profile may be used as markers for identification of the mutants/genotypes. The research results about the biochemical characteristics of desi chickpea varieties are expected to provide guidelines for the researchers confronted with the need to use such typical food seeds in India as well as in the rest of the world.

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