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Asian Journal of Biochemistry

Year: 2011  |  Volume: 6  |  Issue: 1  |  Page No.: 98 - 103

Methaemoglobin Content and NADH-methaemoglobin Reductase Activity of Three Human Erythrocyte Genotypes

P.C. Chikezie

Abstract

The aim of the present study was to ascertain methaemoglobin concentrations and levels of NADH-methaemoglobin reductase activity of three human erythrocyte genotypes, namely, HbAA, HbAS and HbSS. The cyanomethaemoglobin reaction was used for the determination of erythrocyte haemolysate methaemoglobin concentration. NADH-methaemoglobin reductase activity was measured by the rate of oxidation of NADH + H+ when, the erythrocyte enzyme was incubated in potassium ferrocyanide {K3Fe(CN)6}. Whereas, methaemoglobin concentrations in the three erythrocyte genotypes was in the range of 1.45±0.13 to 2.50±0.43%, in the order: HbAS<HbAA<HbSS, levels of NADH-methaemoglobin reductase activity ranged between 8.86±2.49 and 14.77±1.47 IU gHb-1, in the order of: HbSS<HbAS<HbAA. However, there was no significant difference (p<0.05) in methaemoglobin concentration and NADH-methaemoglobin reductase activity between HbAA and HbAS erythrocytes. The results showed a relationship between erythrocyte NADH-methaemoglobin reductase activity and methaemoglobin concentration.

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