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Asian Journal of Biochemistry

Year: 2011  |  Volume: 6  |  Issue: 4  |  Page No.: 347 - 356

Synergistic Effects of Glutathione and Vitamin E on ROS Mediated Ethanol Toxicity in Isolated Rat Hepatocytes

Ankush Gupta, Sangram Singh, Farrukh Jamal, Som Nath, Sudhir Mehrotra and Bechan Sharma


In this study, the potential synergistic relationship between reduced glutathione and vitamin E on ethanol toxicity in isolated rat hepatocytes were examined. It was observed that treatment of ethanol (5-25 mM) caused decrease in cell viability but in presence of Rotenone, cell viability decreased to a greater extent. Inhibitory concentration (IC50) was found to be 20 mM of ethanol treated with rotenone (10 μM) for 2 h. Treatment of ethanol with rotenone (20 mM±10 μM) caused increase in lipid peroxidation, increase of lactate dehydrogenase and decrease in reduced glutathione content. Reduced glutathione and α-tocopherol, alone and in combination, were added to hepatocyte suspension in an attempt to protect cells against alcohol induced cell damage. Pretreatment with reduced glutathione and vitamin E independently, increased the cell viability to 78.97 and 70.42%, respectively, but a combination of reduced glutathione and vitamin E increased cell viability to 92.12%. Vitamin E reduced lipid peroxidation by 41.32% but in combination with reduced glutathione decreased lipid peroxidation by 61.15%. The results suggest an interaction between GSH and Vitamin E in protecting ethanol induced hepatocytes damage.

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