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Asian Journal of Biochemistry
Year: 2010  |  Volume: 5  |  Issue: 1  |  Page No.: 33 - 39

Toxicity Study of Malaysian Rubber (Hevea brasiliensis) Seed Oil as Rats and Shrimps Tests

Bashar Mudhaffar Abdullah and Jumat Salimon    

Abstract: The lipid fraction of Malaysian rubber seed was extracted and analyzed for toxicological compounds such as linamarin. Analytical methods (rat’s toxicological test and brine shrimps test) were applied to determine the presence of such compounds. Malaysian rubber seed oil was extracted using different solvents (hexane and chloroform+methanol). The oil was found not to contain unusual fatty acids and it is rich in essential unsaturated fatty acids, particularly linoleic (37.28±0.10%) and linolenic (19.22±0.21%). Malaysian rubber seed oil showed no toxic potential toward the rats. Bioassay experiments, in which shrimps were used as test organisms, were performed to evaluate the toxicity of linamarin extracted from the oil. The median lethal concentration (LC50) was found to be 211.70%. The results showed no acute toxicity effects, probably since Malaysian rubber seed oil was not found to contain any hazardous linamarin.

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