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Asian Journal of Biochemistry
Year: 2007  |  Volume: 2  |  Issue: 6  |  Page No.: 395 - 401

Effects of Ascorbic Acid Treatment to Grapevine Rootstock-1616C and Vitis vinifera L. cvs. Razaki

Ozlem Yildirim    

Abstract: The glutathione contents, ascorbic acid, nitric oxide, lipid peroxide levels and the activities of free radical scavenging enzymes were examined using five different concentrations of ascorbic acid treated Razaki cultivar and 1616C rootstock. Reduced glutathione was increased with ascorbic acid treatment after seven day in both samples with respect to controls. Similar results were shown in ascorbic acid, nitric oxide and thiobarbituric acid reactive substance levels, while some of these changes were dose dependent. The catalase activity was very low and it increased with increasing ascorbic acid concentration almost in Razaki cultivar and 1616C rootstock. Maximum glutathione peroxidase activity was observed at the end of second day and 10 mg L-1 ascorbic acid treatment for Razaki cultivar. The glutathione peroxidase activity was decreased in 1616C rootstock with respect to controls. Superoxide dismutase activities in these samples were significantly decreased at all concentrations of ascorbic acid and studied times.

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