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Asian Journal of Animal and Veterinary Advances
Year: 2018  |  Volume: 13  |  Issue: 2  |  Page No.: 197 - 200

Live Bacteria in Clots from Bovine Clinical Mastitis Milk with No Growth in Conventional Culturing

Yasunori Shinozuka, Teppei Morita, Aiko Watanabe and Kazuhiro Kawai    

Abstract: Background and Objective: Although screening of clinical mastitic milk for bacteria is necessary for evidence-based antibiotic treatment and prevention of mastitis, these milk samples are frequently culture negative. The purpose of the present study was to evaluate clots from culture-negative mastitic milk for the presence of viable bacteria in an attempt to unravel the reason for the no-growth (NG) status of mastitic milk. Methodology: A total of 449 clinical mastitic milk samples were enrolled, visual inspections were performed to confirm the presence of clots in milk samples and specimens were subjected to bacterial culturing by conventional methods. Milk samples that were NG by primary culture were re-cultured. Furthermore, clots in NG milk samples were recovered aseptically and cultured. Bacteria isolated from milk or clots were identified using biological methods. Results: In primary culture, NG was detected in 96 out of the 449 samples tested (21.4%). The proportion of mastitic milk with clots was significantly higher in NG milk (45.1%, 78/173) than in growth (G) milk (6.5%, 18/276). In the secondary culture of NG milk and its clots, the culture-positive rate of clots from NG milk (53.8%, 42/78) was significantly higher than that from NG milk (34.4%, 33/96). Although no differences were found in other pathogens, the isolation rate of coliforms from clots (20.8%, 20/78) was more than two times that from milk (8.3%, 8/96). Conclusion: These findings suggested that elimination of mastitic bacteria by the host immune system may be a cause of culture-negative mastitic milk and that this pattern is often observed upon infection with coliforms.

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