Molybdenum Salts Possess Potent Angiogenic Modulatory Properties: Validation on Chorioallantoic Membrane (CAM) of Chicken
Objective: Angiogenesis plays critical and essential role in various physiological processes of animals and humans. Present study reports the potential angiogenic modulatory effects of the two different molybdenum salts [molybdenum trioxide (MoO3) and sodium molybdate dihydrate (Na2MoO4.2H2O)] on chorioallantoic membrane (CAM) of embryonated chicken eggs. Methodology: The three groups of the embryonated chicken eggs (1 control and 2 treated groups) were taken and 200 μL of 0.5 M of each of MoO3 and Na2MoO4.2H2O were directly introduced on to the CAM. The resealed eggs were incubated for 72 h in a humid incubator chamber at 37±1°C. Then the eggs were opened to observe the gross and histopathological alterations for angiogenesis modulation. Results: Gross examination revealed reduced number of secondary and tertiary blood vessels in MoO3 treated group, while Na2MoO4.2H2O treated group showed reduction in number of blood vessels with occasional haemorrhages. Histopathological analysis indicated pro-angiogenic effect of MoO3, with presence of numerous mesodermal blood vessels with normal CAM tissue architecture. However, Chorionic Ectoderm (CE) was absent at few places, with intact CE at most places, indicating requirement of critical dose optimization. The Na2MoO4.2H2O was observed as anti-angiogenic, causing inflammation in CAM tissue with hemorrhage and thus cant be used for therapeutics. Conclusion: The pro-angiogenic properties of MoO3 can be explored to treat ailments related to insufficient angiogenesis like coronary artery disease, chronic wounds, stroke and myocardial infarction. However, to avoid any kind of side effects, further qualitative and quantitative analysis and critical dose determination is required before going for clinical trials.
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