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Asian Journal of Animal and Veterinary Advances
Year: 2017  |  Volume: 12  |  Issue: 1  |  Page No.: 24 - 30

Chemical Composition and Antibacterial Activity of Cinnamomum aromaticum Essential Oil Against Four Enteropathogenic Bacteria Associated with Neonatal Calve’s Diarrhea

Selles Sidi Mohammed Ammar, Kouidri Mokhtaria, Ait Amrane Amar, Belhamiti Belkacem Tahar, Drideche Moulay, Hammoudi Si Mohamed and Boukraa Laid    

Abstract: Objective: The aim of this study was to investigate the chemical composition and the in vitro antibacterial activity of Cinnamomum aromaticum essential oil against four strains of enteropathogenic bacteria isolated from neonatal calve’s diarrhea namely Escherichia coli F5, Escherichia coli F17, Kluyvera spp. and Klebsiella spp. Methodology: The essential oil was extracted by hydrodistillation process. The composition was determined by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detector (GC-FID). The agar incorporation method was used to determine the antimicrobial activity of Cinnamomum aromaticum essential oil against four strains of enteropathogenic bacteria isolated from neonatal calve’s diarrhea including Escherichia coli F5, Escherichia coli F17, Kluyvera spp. and Klebsiella spp. Results: The results of the study revealed an average yield of the essential oil of 1.46±0.05% (w/w). About 89 components were identified and quantified. E-cinnamaldehyde was the major compound of the studied essential oil (94.67%). The results showed that the tested essential oil exhibited an antibacterial activity against all tested bacteria at a Minimum Inhibitory Concentration (MIC) of 0.625 μL mL–1. Conclusion: The present investigation revealed that C. aromaticum essential oil is potentially good source of antibacterial agents and could be used as against the tested strains causing diarrhea in calves.

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