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Asian Journal of Animal and Veterinary Advances
Year: 2017  |  Volume: 12  |  Issue: 3  |  Page No.: 152 - 160

Characterization of Quinolone Resistance of Pseudomonas aeruginosa Isolated from Pet Chinese Stripe-necked Turtles (Ocadia sinensis)

B.C.J. De Silva, S.H.M.P. Wimalasena, Sabrina Hossain, H.N.K.S. Pathirana and Gang-Joon Heo    

Abstract: Background and Objective: Infections of antibiotic-resistant bacteria in both human and animals own a great significance. The current study aimed to determine the quinolone susceptibility and the genetic characteristics of quinolone resistance of 20 strains of Pseudomonas aeruginosa isolated from pet Chinese stripe-necked turtles (Ocadia sinensis ). Methodology: Susceptibility of four antimicrobials including nalidixic acid, ciprofloxacin, ofloxacin and levofloxacin was examined. The PCR was carried out to amplify Quinolone Resistance Determining Region (QRDR) and to screen Plasmid Mediated Quinolone Resistance (PMQR) genes. Results: All tested isolates were resistant to nalidixic acid (100%), but none of the isolates show complete resistance to other tested antimicrobials. Two isolates showed intermediate resistance to ciprofloxacin (5%) and ofloxacin (5%), one each in Minimum Inhibitory Concentration (MIC) test. The qnrB gene was identified in one isolate (5%) and qnrS in three isolates (15%). The PCR assay could amplify aac(6’)-Ib gene from 8 isolates (40%) and none of them harbored aac(6’)-Ib-cr variant. Sequences obtained by amplifying gyrA and parC regions did not show any point mutation in QRDR. Neighbor-joining phylogenetic tree for gyrA indicated three distinct clads comprising first, current study isolates, second, clinical isolates of human and dogs and third, isolates from soil and water. Conclusion: All results suggest that studied strains of P. aeruginosa are less resistant to quinolones and are genetically more conserved with regards to gyrA gene region.

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