Molecular Characterization of Arcobacter Isolates Using Randomly
Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR)
In modern years, Arcobacters are reflected as potential emerging food-borne
zoonotic entero-pathogens. Arcobacter species displayed a wide variety
of genetic diversity. The study was carried out to genotype and find molecular
heterogeneity of Arcobacter spp. (Arcobacter butzleri, A. cryaerophilus
and A. skirrowii), isolated from different sources from Bareilly region,
Uttar Pradesh, India by using randomly amplified polymorphic DNA - polymerase
chain reaction (RAPD-PCR). RAPD-PCR was performed using genomic DNA of Arcobacter
isolates (n = 56; 33 A. butzleri, 20 A. cryaerophilus, 3 A.
skirrowii; recovered from chicken meat, pork, sheep faeces, goat faeces,
poultry intestinal contents and human diarrhoeal stool samples) as template
by employing two published primers. The RAPD profiling for primer 1 (HLWL85)
yielded number of bands rangeing between 2-8 (500-3100 bp). Out of 56 isolates,
54 showed bands giving a typeability of 96.4%. These 54 typable strains were
grouped to 35 types and giving discriminatory power of 0.9762. Primer 2 (OPA-11)
yielded RAPD-PCR profiles comprising of 2-7 bands (210-2800 bp). Out of total
56 isolates, 54 were typable with a discriminatory power of 0.9336. This is
the first report from India regarding RAPD profiling of Arcobacter spp.
This study reveals epidemiological relationship of Arcobacter isolate
from various sources and will help to design suitable prevention and control
strategies for this important pathogen having public health significance.
Cited References Fulltext