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Asian Journal of Animal and Veterinary Advances
Year: 2013  |  Volume: 8  |  Issue: 5  |  Page No.: 754 - 760

Sequencing and Homological Analysis of Silver Fox TYR Gene

Zhengzhu Liu, Yuanfang Gong, Lingxin Duan, Minshan Feng, Wenjin Zhu, Muxiang Ge, Xierong Liu, Junsheng Zhao and Xinling Guo    

Abstract: To explore the sequence structure of fox TYR gene and it’s mechanism to regulate the pelage color’s dividing. In this study, the partial DNA sequence (1370 bp) of silver fox TYR gene was obtained by PCR amplification and direct sequencing. This sequence contained the intron 1 of 368 bp, the complete exon 2 of 217 bp and the intron 2 of 785 bp and GC content was 40.5%. Based on the sequences of TYR gene exon 2, the homological analysis and the construction of un-rooted phylogenetic tree were carried out among silver fox and the other 16 species by using DNAMAN software and Clustalx (1.83) software, respectively. The homological analysis showed that silver fox had the closest genetic relationship with dog and the closer genetic relationship with domestic cat and domestic ferret. The cluster result of un-rooted phylogenetic tree was similar to that of the homological analysis. The length of TYR gene exon 2 were 217 bp for all analyzed species, which indicated that TYR gene exon 2 were highly conservative in phylogeny. The above results can provide the important biological information for further researching the development and regulation mechanism of fox coat color which result from TYR gene.

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