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Asian Journal of Animal and Veterinary Advances
Year: 2013  |  Volume: 8  |  Issue: 2  |  Page No.: 355 - 361

Antibacterial Activity of Neem (Azadirachta indica) Leaves on Vibrio spp. Isolated from Cultured Shrimp

Sanjoy Banerjee, Lee Mei Kim, Mohamed Shariff, Helena Khatoon and Fatima Md. Yusoff    

Abstract: The use of antibiotics in aquaculture to treat infections has resulted in the development of resistant strains which have rendered antibiotic treatment ineffective. Therefore, alternative antibacterial materials must be found. Extracts of neem tree (Azadirachta indica) leaves were tested against Vibrio parahaemolyticus and Vibrio alginolyticus isolated from cultured shrimp. Aqueous extract of neem leaves did not produce any inhibitory zone while the neem juice produced inhibitory zone that showed linear relationship to the concentration of neem juice on both bacteria. The Minimum Inhibitory Concentration (MIC) for V. parahaemolyticus and V. alginolyticus was 3.13 and 6.25%, respectively. The Minimum Bactericidal Concentration (MBC) for V. parahaemolyticus and V. alginolyticus was 12.50 and 25.00%, respectively. It is concluded that neem juice is an antibacterial agent and is useful for inhibition of vibrios in shrimp.

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