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Asian Journal of Animal and Veterinary Advances

Year: 2011  |  Volume: 6  |  Issue: 7  |  Page No.: 654 - 666

Construction of a Recombinant Fowlpox Virus Expressing the E0/E2 Proteins of Classical Swine Fever Virus and its Evaluation in Experimental Animals

Kang-Kang Guo, Wei-Ming Zhang, Yan-Ming Zhang, Peng-Bo Ning, Jing-Yu Wang and Miao-Tao Zhang

Abstract

Classical Swine Fever (CSF) is a hemorrhagic disease of pigs caused by virulent strains of Classical Swine Fever Virus (CSFV). Vaccination is a useful tool for preventing and controlling CSF. In this study the protective antigens E0 and E2 genes of CSFV were cloned and inserted into a fowlpox virus vector to construct a recombinant plasmid. We transfected this plasmid into chicken embryo cells infected with the fowlpox virus FV282 to package and propagate the recombinant fowlpox virus FV282-E0-E2. Specific anti-CSFV antibodies were detected with enzyme-linked immunosorbent assay in FV282-E0-E2 immunized experimental mice and pigs. Moreover, virus challenge experiments were conducted in experimental pigs immunized with FV282-E0-E2. Immune protection was evaluated in experimental pigs infected with virulent CSFV by observing clinical manifestations and post-mortem examination. The results showed that recombinant fowlpox virus FV282-E0-E2 was obtained after ten cycles of blue plaque selection. The special anti-CSFV antibodies were detected in the experimental mice and pigs. The immunized pigs could resist the attack of the virulent CSFV strain. The recombinant fowlpox virus FV282-E0-E2 represents a potential candidate for the development of a genetic engineered vaccine for the future prevention of CSF.

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