Abstract: Interferon regulatory factor 7 (IRF-7) plays a crucial role in virus-induced activation of interferon-alpha/beta transcription and innate immunity. In order to evaluate its effect on cytokine traits in pig, IRF7 gene was chosen as a candidate gene to investigate in pig populations. SNP was identified by sequencing and PCR-RFLP method. Cytokine traits including IFN-γ and IL10 concentration in serum were measured when pigs were at the age of 20 and 35 days, respectively. The further association analysis between SNP genotypes and cytokine traits were conducted in three breeds including Large White, Landraces and Songliao Black, the later one is Chinese indigenous breed. The results indicated that the SNP of IRF7 gene had a highly significant effect on the level of IL10 (day 20) (p = 0.047), IFN-γ (day 35) in serum (p = 0.031) and the ratio of IFN-γ to IL10 (day 35) in serum (p = 0.035). The study suggested that IRF7 gene could be regarded as a molecular marker gene for genetic selection of cytokine traits in the further disease resistance breeding.